Polyclonal Antibody Production against Somatotropin to Estimate Somatotropin Levels in Buffaloes by Elisa
نویسنده
چکیده
The aim of the study was to produce sensitive and specifi c polyclonal antibodies against somatotropin (ST) to be used for standardization of an ELISA to measure growth hormone (GH) level in serum and milk of lactating buffaloes. Recombinant bovine somatotropin (rbST), kindly donated by NHPP, California, USA was injected both in chicken and rabbit emulsifi ed with Freund’s complete adjuvant followed by injection with Freund’s incomplete adjuvant. Polyclonal antibodies in sera were detected by indirect ELISA and separated by the salt precipitation method. Immunoglobulins were purifi ed by ion exchange chromatography on a DEAE Sepharose column followed by affi nity chromatography. Purity of anti rbST polyclonal antibodies were determined by SDS-PAGE analysis and western blot. Antibody content of the sample was determined by Lowry’s method.
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